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Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.
Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S
Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80008pX330-COSMC-KOgRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.
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80009pX330-MGAT1-KOgRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.
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80010pX330-UGCG-KOgRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.
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