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Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes.
Pritchard CEJ, Kroese LJ, Huijbers IJ
Methods Mol Biol. 2017;1642:21-35. doi: 10.1007/978-1-4939-7169-5_2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
82580pX330-Puro-ccdBA modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette
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82581bbCas9pluspAAAThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
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