Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes.
Pritchard CEJ, Kroese LJ, Huijbers IJ
Methods Mol Biol. 2017;1642:21-35. doi: 10.1007/978-1-4939-7169-5_2. PubMed Article
Pritchard CEJ, Kroese LJ, Huijbers IJ
Methods Mol Biol. 2017;1642:21-35. doi: 10.1007/978-1-4939-7169-5_2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 82580 | pX330-Puro-ccdB | A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette |
| 82581 | bbCas9pluspAAA | The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA |