Skip to main content
Holiday Schedule: Addgene will be closed December 24 - December 31. Order processing and shipping will resume on January 3, 2022. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Dorus Gadella Lab: Gadella lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
98832CytERM-mKOkappaIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
Loading...
98833CytERM-mTurquoise2In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
Loading...
98834CytERM-dTomatoIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
Loading...
112936LSS-SGFP2-aTubVisualization of microtubules
Loading...
112937LSS-SGFP2_H2AVisualization of histones
Loading...
112938LifeAct-LSS-SGFP2-N1Visualization of F-actin
Loading...
112939pRSETb-LSS-SGFP2Bacterial expression vector for LSS-SGFP2
Loading...
112940CytERM-LSS-SGFP2In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
Loading...
112941pRSETb-LssmsfGFPBacterial expression vector for LSS-msfGFP
Loading...
112942pRSETb-LSS-mEGFPBacterial expression vector for LSS-EGFP
Loading...
112943mCherry-LSS-SGFP2Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET
Loading...
112944mCherry-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
Loading...
112945mScarlet-I-LSS-SGFP2Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
Loading...
112946mScarlet-I-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
Loading...
112947RhoA sensor (LSS-SGFP2-Scarlet-I)RhoA FRET biosensor
Loading...
112948RhoA sensor (LSS-SGFP2-mCherry)RhoA FRET biosensor
Loading...
112949pmCherry-Ggamma2mCherry-Ggamma2 fusion
Loading...
112950mScarlet-I-Ggamma2mScarlet-I-Ggamma2 fusion
Loading...
112951Gaq-LSS-SGFP2Galphaq tagged with LSS-SGFP2
Loading...
112952RhoA sensor (mScarlet-I_10_LSS-SGFP2_10)RhoA FRET biosensor
Loading...
112953pTfR-mTurquoise2In vivo visualization of Transferrin receptors (can be used for colocalization studies)
Loading...
112954pTfR-mScarlet-IIn vivo visualization of Transferrin receptors (can be used for colocalization studies)
Loading...
112958CytERM_3xmTurquoise2 In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
Loading...
130509pDRESS_mTurquoise2_spatial-linker-P2A_mScarletexpresses mScarlet
Loading...
130510pDRESS_mTurquoise2_spatial-linker-P2A_mScarletIexpresses mScarlet1
Loading...
137809PiggyBac-G13-sensorA FRET sensor for G13 activation, encoded on a single plasmid
Loading...
176092iRFP670 - H2Anuclear marker
Loading...
176093miRFP670 - H2ANucelear marker
Loading...
176096H2A-mTurquoise-RHOG-QL-deltaCAAXConstitutively active, nuclear localized RhoG
Loading...
176099dimericTomato-1xwGBDVisualization of active Cdc42
Loading...
176100mTurquoise2-3xrGBDVisualization of active Rho
Loading...
176103SspB-mCherry-DHPH-ITSN1Heterodimerization with iLID, Cdc42 activation
Loading...
176104SspB-mCherry-DHPH-TIAM1Heterodimerization with iLID, Rac1 activation
Loading...
176105LifeAct-HaloTagF-actin marker
Loading...
176108HaloTag-3xrGBDVisualization of active Rho
Loading...
176109HaloTag-2xrGBDVisualization of active Rho
Loading...
176110H2A-mTurquoise-RhoJQ79L-deltaCaaxConstitutively active, nucelar localized RhoJ
Loading...
176111SspB-mCherry-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
Loading...
176112SSpB-mCherry-p63DHHeterodimerization with iLID, RhoA activation
Loading...
176113SspB-HaloTag-DHPH-ITSN1Heterodimerization with iLID, Cdc42 activation
Loading...
176114SspB-HaloTag-DHPH-TIAM1Heterodimerization with iLID, Rac1 activation
Loading...
176115SspB-HaloTag-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
Loading...
176116SSpB-HaloTag-p63DHHeterodimerization with iLID, RhoA activation
Loading...
176117SspB-iRFP-DHPH-ITSN1Heterodimerization with iLID, Cdc42 activation
Loading...
176118SspB-iRFP-DHPH-TIAM1Heterodimerization with iLID, Rac1 activation
Loading...
176119SspB-iRFP-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
Loading...
176120SSpB-iRFP-p63DHHeterodimerization with iLID, RhoA activation
Loading...
176121TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaXConstitutivley active, mitochondrial localized Cdc42
Loading...
176122TOMM20-mTurquoise-RhoG-QL-deltaCaaXConstitutively active, mitochondiral localized RhoG
Loading...
176123TOMM20-mTurquoise-RhoA-G14V-deltaCaaXConstitutively active, mitochondrial localized RhoA
Loading...
176124TOMM20-mTurquoise-Rac1-G12V-deltaCaaXConstitutively active, mitochondrial localized Rac1
Loading...
176125Lck-mTurquoise2-iLIDPlasma membrane localized iLID
Loading...
176126pLV-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation
Loading...
176127pLV-SspB-HaloTag-TIAM1-DHPHHeterodimerization with iLID, Rac1 activation
Loading...
176128pLV-SspB-HaloTag-ITSN1-DHPHHeterodimerization with iLID, Cdc42 activation
Loading...
176130pLV-Lck-FRB-mTurquoise2Plasma membrane localized FRB, rapamycin heterodimerization
Loading...
176133pEntr1a-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation
Loading...
176134pEntr1a-SspB-HaloTag-TIAM1-DHPHHeterodimerization with iLID, Rac1 activation
Loading...
176135pEntr1a-mcherry-FKBP-TIAM1(DHPH)Heterodimerization with FRB (rapamycin), Rac1 activation
Loading...
176136pEntr1a-wGBD-mTurquoise2-wGBDVisualization of active Cdc42
Loading...
176137pEntra1a-mTurquoise2-3xrGBDVisualization of active Rho
Loading...
176138pLV-mCherry-FKBP-TIAM1(DHPH)Heterodimerization with FRB (rapamycin), Rac1 activation
Loading...