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Dorus Gadella Lab: Gadella lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
98832CytERM-mKOkappaIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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98833CytERM-mTurquoise2In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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98834CytERM-dTomatoIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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112936LSS-SGFP2-aTubVisualization of microtubules
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112937LSS-SGFP2_H2AVisualization of histones
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112938LifeAct-LSS-SGFP2-N1Visualization of F-actin
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112939pRSETb-LSS-SGFP2Bacterial expression vector for LSS-SGFP2
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112940CytERM-LSS-SGFP2In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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112941pRSETb-LssmsfGFPBacterial expression vector for LSS-msfGFP
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112942pRSETb-LSS-mEGFPBacterial expression vector for LSS-EGFP
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112943mCherry-LSS-SGFP2Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET
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112944mCherry-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
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112945mScarlet-I-LSS-SGFP2Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
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112946mScarlet-I-T2A-LSS-SGFP2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
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112947RhoA sensor (LSS-SGFP2-Scarlet-I)RhoA FRET biosensor
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112948RhoA sensor (LSS-SGFP2-mCherry)RhoA FRET biosensor
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112949pmCherry-Ggamma2mCherry-Ggamma2 fusion
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112950mScarlet-I-Ggamma2mScarlet-I-Ggamma2 fusion
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112951Gaq-LSS-SGFP2Galphaq tagged with LSS-SGFP2
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112952RhoA sensor (mScarlet-I_10_LSS-SGFP2_10)RhoA FRET biosensor
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112953pTfR-mTurquoise2In vivo visualization of Transferrin receptors (can be used for colocalization studies)
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112954pTfR-mScarlet-IIn vivo visualization of Transferrin receptors (can be used for colocalization studies)
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112958CytERM_3xmTurquoise2 In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
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130509pDRESS_mTurquoise2_spatial-linker-P2A_mScarletexpresses mScarlet
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130510pDRESS_mTurquoise2_spatial-linker-P2A_mScarletIexpresses mScarlet1
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137809PiggyBac-G13-sensorA FRET sensor for G13 activation, encoded on a single plasmid
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176092iRFP670 - H2Anuclear marker
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176093miRFP670 - H2ANucelear marker
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176105LifeAct-HaloTagF-actin marker
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176111SspB-mCherry-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
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176115SspB-HaloTag-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
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176119SspB-iRFP-DHPH1-TRIOHeterodimerization with iLID, Rac1 and RhoG activation
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176126pLV-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation
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176133pEntr1a-SspB-HaloTag-TRIO-DHPH1Heterodimerization with iLID, Rac1 and RhoG activation
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Antibodies from Article