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Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.
Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
84354CyRFP1-C1Expresses CyRFP1 fluorescent protein in an empty backbone
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84355mCyRFP1-C1Expresses mCyRFP1 fluorescent protein in an empty backbone
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84356pCAG-CyRFP1Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter
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84357AAV-FLEX-CAG-CyRFP1Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)
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84358mCyRFP1-RhoAfusion protein of RhoA, FRET donor
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84359mMaroon-RTK-mMaroonFRET acceptor for RhoA donor, flanked with the far-red FP mMaroon
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84360mCyRFP1-Cdc42fusion protein of Cdc42, FRET donor
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84361mMaroon-PAK-mMaroonFRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon
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84486mCyRFP1-mMaroonDirect FRET pair Fusion mCyRFP1-mMaroon
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84545pNCS-mCyRFP1Bacterial expression of mCyRFP1
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84546pNCS-CyRFP1Bacterial expression of CyRFP1
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