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A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G
Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
83073pGST-DHFR MmExpresses the full length DHFR Mm in E.coli
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83232pGEN25-Ub HspHis-par2-Ub Hs
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83233pCOG75-Sem1 ScpCDF-His6-MBP-Sem1 Sc
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83234pGEN6-Ubc5 ScpHis-par2-UbHs/Ubc5Sc/ Uba1Ta
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83235pSc-His6-Sem1ScpGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast
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83236pCOG40-HRS (257-276) HspGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli
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Antibodies from Article