pSc-His6-Sem1Sc
(Plasmid #83235)

• Purpose: pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast
• Depositing Lab: Gali Prag
• Publication: Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3.

Backbone

• Vector backbone: pGREG600
• Backbone size w/o insert (bp): 7176
• Total vector size (bp): 7476
• Modifications to backbone: To create the pScHis6Sem1 the GFP sequence was removed from the pGREG600 vector by Gibson assembly (Gibson et al, 2009). Then the cDHA of yeast Sem1 was sub-cloned in frame with His6 under the Gal promoter.
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418), URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sem 1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 270
• Entrez Gene: SEM1 (a.k.a. YDR363W-A, DSS1, HOD1)
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry