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Conformational control of DNA target cleavage by CRISPR-Cas9.
Sternberg SH, LaFrance B, Kaplan M, Doudna JA
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87346pSHS212Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid
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87348pSHS293D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct
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87350pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variantS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct
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87354pSHS248S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct
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