Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects.
Ooi J, Langley SR, Xu X, Utami KH, Sim B, Huang Y, Harmston NP, Tay YL, Ziaei A, Zeng R, Low D, Aminkeng F, Sobota RM, Ginhoux F, Petretto E, Pouladi MA
Cell Rep. 2019 Feb 26;26(9):2494-2508.e7. doi: 10.1016/j.celrep.2019.02.008. PubMed Article
Ooi J, Langley SR, Xu X, Utami KH, Sim B, Huang Y, Harmston NP, Tay YL, Ziaei A, Zeng R, Low D, Aminkeng F, Sobota RM, Ginhoux F, Petretto E, Pouladi MA
Cell Rep. 2019 Feb 26;26(9):2494-2508.e7. doi: 10.1016/j.celrep.2019.02.008. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92243 | USPQ-HTT-TALEN1 | TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2 |
| 92244 | USPQ-HTT-TALEN2 | TALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1 |
| 92245 | DSPQ-HTT-TALEN1 ZEO | TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD |
| 92246 | DSPQ-HTT-TALEN2 BSD | TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN |
| 92247 | pJOP-HTT-HR30Q | HTT donor DNA with 30Q for homologous recombination-mediated gene modification |
| 92248 | pJOP-HTT-HR45Q | HTT donor DNA with 45Q for homologous recombination-mediated gene modification |
| 92249 | pJOP-HTT-HR65Q | HTT donor DNA with 65Q for homologous recombination-mediated gene modification |
| 92250 | pJOP-HTT-HR81Q | HTT donor DNA with 81Q for homologous recombination-mediated gene modification |