Isolation of gene-edited cells via knock-in of short glycophosphatidylinositol-anchored epitope tags.
Zotova A, Pichugin A, Atemasova A, Knyazhanskaya E, Lopatukhina E, Mitkin N, Holmuhamedov E, Gottikh M, Kuprash D, Filatov A, Mazurov D
Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.1038/s41598-019-40219-z. PubMed Article
Zotova A, Pichugin A, Atemasova A, Knyazhanskaya E, Lopatukhina E, Mitkin N, Holmuhamedov E, Gottikh M, Kuprash D, Filatov A, Mazurov D
Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.1038/s41598-019-40219-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89704 | pJet-CMV-CD52-bglpA | Human CD52 expression plasmid with short polyA |
| 89705 | pJet-CMV-CD5Flag2-bglpA | Expression plasmid for Flag embedded into human CD52 with short polyA |
| 96962 | pJet-CMV-CD5HA2-bglpA | Expression plasmid for HA-tag embedded into human CD52 with short polyA |
| 110645 | pJet-CMV-CD5cmyc2-bglpA | Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA |
| 110654 | pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA | Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron |
| 110655 | pUCHR-mClover-smAID-P2A-CD5HA2-bglpA | Lentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degron |
| 110656 | pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA | Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron |
| 110657 | pUCHR-CD5Flag2-P2A-smAID-mClover | Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron |
| 110658 | pUCHR-CD5HA2-P2A-smAID-mClover | Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron |
| 110661 | pUCHR-osTIR1-IRES-GFP | Lentiviral vector for stable expression of osTIR1 and selection with GFP |