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Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
89232pCsy_complexPlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site
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89233pAcrF1Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site
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89234pAcrF2Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site
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89244pCRISPR_DMS3g24Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3
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Recombinant Antibodies from Article