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Addgene

pAcrF2
(Plasmid #89234)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89234 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p15TV-L

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AcrF2
  • Alt name
    Gene 30 from bacteriophage D3112
  • GenBank ID
    UniProt: Q6TM72
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Joseph Bondy-Denomy (University of California San Francisco ) and Alan Davidson (University of Toronto)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcrF2 was a gift from Blake Wiedenheft (Addgene plasmid # 89234 ; http://n2t.net/addgene:89234 ; RRID:Addgene_89234)
  • For your References section:

    Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex. Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B. Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012. 10.1016/j.cell.2017.03.012 PubMed 28340349