A Mutant Sumo Facilitates Quick Plasmid Construction for Expressing Proteins with Native N-termini After Tag Removal.
Zhang Y, Fan Y
Mol Biotechnol. 2017 May;59(4-5):159-167. doi: 10.1007/s12033-017-9998-6. PubMed
Zhang Y, Fan Y
Mol Biotechnol. 2017 May;59(4-5):159-167. doi: 10.1007/s12033-017-9998-6. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87797 | pTS1 | For constructing SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning. |
| 87798 | pTS2 | For constructing SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning. |
| 87799 | pTMS1 | For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning. |
| 87800 | pTMS2 | For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning. |
| 87801 | pTNS1 | For constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning. |
| 87802 | pTNS2 | For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning. |