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(Plasmid #87802)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87802 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    EMD Millipore
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • Strep II (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Tags / Fusion Proteins
    • Strep II (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The constructs contain a DNA sequence which encodes a mutant sumo from yeast. I had the coding sequence synthesized by a commercial vendor.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional reference: Zhang, Y.; Du, W.X.; Fregevu, C.; Kothary, M.H.; Harden, L; McHugh, T.H. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J. Agric Food Chem 2014, 62, 12695-700. PMID: 25437489

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTNS2 was a gift from Yu-Zhu Zhang (Addgene plasmid # 87802 ; ; RRID:Addgene_87802)
  • For your References section:

    A Mutant Sumo Facilitates Quick Plasmid Construction for Expressing Proteins with Native N-termini After Tag Removal. Zhang Y, Fan Y. Mol Biotechnol. 2017 May;59(4-5):159-167. doi: 10.1007/s12033-017-9998-6. 10.1007/s12033-017-9998-6 [pii] PubMed 28349302