Functional dissection of NEAT1 using genome editing reveals substantial localisation of the NEAT1_1 isoform outside paraspeckles.

Li R, Harvey AR, Hodgetts SI, Fox AH
RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
97081	pX330-NEAT1pr_v1	Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene
97082	pX330-NEAT1pr_v2	Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene
97083	pX330-NEAT1m_v1	Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform
97084	pX330-GFP-SFPQ	Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.
97085	pX335-NEAT1_IS_v1	Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
97086	pX335-NEAT1_IS_v2	Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
97088	pYFP-NEAT1pr_v2-RT	Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.
97089	pYFP-NEAT1m_v1-RT	Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
97090	pUC57-GFP-SFPQ-RT	Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.
97091	pYFP-NEAT1_IS_RT	Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.