PurposeEncodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||97091||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneNEAT1 (a.k.a. LINC00084, NCRNA00084, TP53LC15, TncRNA, VINC)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The entire repair template is synthesized and then cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y. Also see original publication for mutations on the repair template.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYFP-NEAT1_IS_RT was a gift from Archa Fox (Addgene plasmid # 97091 ; http://n2t.net/addgene:97091 ; RRID:Addgene_97091)
For your References section:Functional dissection of NEAT1 using genome editing reveals substantial localisation of the NEAT1_1 isoform outside paraspeckles. Li R, Harvey AR, Hodgetts SI, Fox AH. RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116. 10.1261/rna.059477.116 PubMed 28325845