Functional dissection of NEAT1 using genome editing reveals substantial localisation of the NEAT1_1 isoform outside paraspeckles.
Li R, Harvey AR, Hodgetts SI, Fox AH
RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116. PubMed Article
Li R, Harvey AR, Hodgetts SI, Fox AH
RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 97081 | pX330-NEAT1pr_v1 | Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene |
| 97082 | pX330-NEAT1pr_v2 | Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene |
| 97083 | pX330-NEAT1m_v1 | Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform |
| 97084 | pX330-GFP-SFPQ | Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene. |
| 97085 | pX335-NEAT1_IS_v1 | Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform. |
| 97086 | pX335-NEAT1_IS_v2 | Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform. |
| 97088 | pYFP-NEAT1pr_v2-RT | Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1. |
| 97089 | pYFP-NEAT1m_v1-RT | Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector. |
| 97090 | pUC57-GFP-SFPQ-RT | Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector. |
| 97091 | pYFP-NEAT1_IS_RT | Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors. |