PurposeEncodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||97088||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneNEAT1 (a.k.a. LINC00084, NCRNA00084, TP53LC15, TncRNA, VINC)
- Promoter CMV
/ Fusion Protein
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The left arm of the repair template was cloned into the AseI site. The right arm was cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYFP-NEAT1pr_v2-RT was a gift from Archa Fox (Addgene plasmid # 97088 ; http://n2t.net/addgene:97088 ; RRID:Addgene_97088)
For your References section:Functional dissection of NEAT1 using genome editing reveals substantial localisation of the NEAT1_1 isoform outside paraspeckles. Li R, Harvey AR, Hodgetts SI, Fox AH. RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116. 10.1261/rna.059477.116 PubMed 28325845