Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution.
Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J
Nat Methods. 2017 Apr;14(4):427-434. doi: 10.1038/nmeth.4221. Epub 2017 Mar 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87704 | Dronpa-DEVD-TagRFPT | Fluorescent biosensor for super-resolution imaging of caspase activity. |
| 87705 | FLINC-AKAR1-CAAX | Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane. |
| 87706 | FLINC-AKAR1(TA)-CAAX | Negative-control mutant of FLINC-AKAR1-CAAX. |
| 87707 | Lyn-FLINC-EKAR1 | Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane. |
| 87708 | Lyn-FLINC-EKAR1(TA) | Negative-control mutant of Lyn-FLINC-EKAR1. |
| 87709 | FKBP-Dronpa | FKBP-tagged Dronpa. |
| 87710 | FRB-TagRFPT-CAAX | FRB-tagged TagRFPT; targeted to plasma membrane. |
| 87711 | Dronpa-FHA1 | Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR). |
| 87712 | PKAsub-TagRFPT-CAAX | Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711). |
| 87740 | PKAsub(TA)-TagRFPT-CAAX | Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711). |