Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
Zhao Y, Boeke JD
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363. PubMed Article
Zhao Y, Boeke JD
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363. PubMed Article
Plasmids from Article
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98404 | pYZ033 | Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1 |
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98405 | pYZ145 | Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion. |
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98406 | pYZ146 | Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe |
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98407 | pYZ149 | Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion. |
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98408 | pYZ164 | Cas9-gRNA his3 plasmid for his3 deletion in S. pombe |
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98409 | pYZ172 | Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion. |
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98410 | pYZ173 | Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe |
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98411 | pYZ155 | Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker |
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98412 | pYZ156 | Pombe Expression Vector - nmt41 promoter with Sp.ura4 marker |
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98413 | pYZ157 | Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker |
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98414 | pYZ158 | Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker |
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98415 | pYZ159 | Pombe Expression Vector - nmt41 promoter with Sp.leu1 marker |
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98416 | pYZ160 | Pombe Expression Vector - nmt81 promoter with Sp.leu1 marker |
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98418 | pYZ165 | Pombe Expression Vector - nmt1 promoter with Sp.his3 marker |
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98419 | pYZ166 | Pombe Expression Vector - nmt41 promoter with Sp.his3 marker |
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98420 | pYZ167 | Pombe Expression Vector - nmt81 promoter with Sp.his3 marker |
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98421 | pYZ174 | Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker |
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98422 | pYZ175 | Pombe Expression Vector - nmt41 promoter with Sp.lys9 marker |
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98423 | pYZ176 | Pombe Expression Vector - nmt81 promoter with Sp.his3 marker |
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98424 | pYZ182 | Pombe Expression Vector - nmt1 promoter with empty BsaI-pad - no marker |
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98425 | pYZ183 | Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker |
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98426 | pYZ184 | Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker |
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98427 | pYZ189 | Pombe General Purpose Vector - with empty BsaI-pad for new potential markers |
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98428 | pYZ190 | Pombe General Purpose Vector - with Sp.ura4 marker |
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98429 | pYZ191 | Pombe General Purpose Vector - with Sp.leu1 marker |
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98430 | pYZ192 | Pombe General Purpose Vector - with Sp.his3 marker |
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98431 | pYZ193 | Pombe General Purpose Vector - with Sp.lys9 marker |
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102686 | pYZ292 | S. pombe gRNA entry vector with Cas9 - ScLEU2 marker |
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102687 | pYZ293 | S. pombe gRNA entry vector with Cas9 - Sp leu1 marker |
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102688 | pYZ294 | S. pombe gRNA entry vector with Cas9 - KanMX marker |
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102689 | pYZ300 | S. pombe gRNA entry vector with Cas9 - Sp his3 marker |
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102690 | pYZ301 | S. pombe gRNA entry vector with Cas9 - Sp lys9 marker |
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