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Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
Zhao Y, Boeke JD
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
98404pYZ033Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
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98405pYZ145Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.
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98406pYZ146Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe
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98407pYZ149Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.
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98408pYZ164Cas9-gRNA his3 plasmid for his3 deletion in S. pombe
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98409pYZ172Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
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98410pYZ173Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe
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98411pYZ155Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker
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98412pYZ156Pombe Expression Vector - nmt41 promoter with Sp.ura4 marker
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98413pYZ157Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker
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98414pYZ158Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker
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98415pYZ159Pombe Expression Vector - nmt41 promoter with Sp.leu1 marker
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98416pYZ160Pombe Expression Vector - nmt81 promoter with Sp.leu1 marker
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98418pYZ165Pombe Expression Vector - nmt1 promoter with Sp.his3 marker
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98419pYZ166Pombe Expression Vector - nmt41 promoter with Sp.his3 marker
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98420pYZ167Pombe Expression Vector - nmt81 promoter with Sp.his3 marker
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98421pYZ174Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker
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98422pYZ175Pombe Expression Vector - nmt41 promoter with Sp.lys9 marker
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98423pYZ176Pombe Expression Vector - nmt81 promoter with Sp.his3 marker
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98424pYZ182Pombe Expression Vector - nmt1 promoter with empty BsaI-pad - no marker
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98425pYZ183Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker
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98426pYZ184Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker
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98427pYZ189Pombe General Purpose Vector - with empty BsaI-pad for new potential markers
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98428pYZ190Pombe General Purpose Vector - with Sp.ura4 marker
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98429pYZ191Pombe General Purpose Vector - with Sp.leu1 marker
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98430pYZ192Pombe General Purpose Vector - with Sp.his3 marker
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98431pYZ193Pombe General Purpose Vector - with Sp.lys9 marker
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102686pYZ292S. pombe gRNA entry vector with Cas9 - ScLEU2 marker
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102687pYZ293S. pombe gRNA entry vector with Cas9 - Sp leu1 marker
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102688pYZ294S. pombe gRNA entry vector with Cas9 - KanMX marker
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102689pYZ300S. pombe gRNA entry vector with Cas9 - Sp his3 marker
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102690pYZ301S. pombe gRNA entry vector with Cas9 - Sp lys9 marker
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