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Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells.

Xie S, Duan J, Li B, Zhou P, Hon GC
Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
89493Lenti-sgRNA(MS2)-puro-barcodeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq
89567Lenti-dCas9-KRAB-blastPlasmid expression dCas9 protein in fusion with KRAB domain

Antibodies from Article