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George Church Lab: Church Lab CRISPR plasmids 2017
Unpublished

Plasmids from Article

ID Plasmid Purpose  
99651pAAV-dSa-VPRExpresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
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99652CRISPR-Sa-TdTom. ReporterRed fluorescent reporter that can be activated by dSa Cas9 activator indicating activity
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99654dSp-p65 Full (1-261)Expresses dSp Cas9 fused to p65 activation domain
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99655dSp-p65 (150-261)Expresses dSp Cas9 fused to truncated p65 activation domain
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99656dSp-p65 (100-261)Expresses dSp Cas9 fused to truncated p65 activation domain
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99657dSp-p65 (200-261)Expresses dSp Cas9 fused to truncated p65 activation domain
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99658dSp-p65 (1-200)Expresses dSp Cas9 fused to truncated p65 activation domain
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99659dSp-p65 (1-150)Expresses dSp Cas9 fused to truncated p65 activation domain
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99660dSp-p65 (1-100)Expresses dSp Cas9 fused to truncated p65 activation domain
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99661dSp-p65 (50-150)Expresses dSp Cas9 fused to truncated p65 activation domain
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99662dSp-Rta Full (1-190)Expresses dSp Cas9 fused to truncated RTA activation domain
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99663dSp-Rta 75-190Expresses dSp Cas9 fused to truncated RTA activation domain
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99664dSp-Rta 125-190Expresses dSp Cas9 fused to truncated RTA activation domain
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99665dSp-Rta 50-175Expresses dSp Cas9 fused to truncated RTA activation domain
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99666dSp-Rta 75-175Expresses dSp Cas9 fused to truncated RTA activation domain
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99667dSp-Rta 100-175Expresses dSp Cas9 fused to truncated RTA activation domain
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99668dSp-Rta 125-175Expresses dSp Cas9 fused to truncated RTA activation domain
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99669dSp-VP64-RTA(75-190)Expresses dSp Cas9 fused to combination of truncated activation domains
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99670dSp-VP64-p65-RTA(75-190)Expresses dSp Cas9 fused to combination of truncated activation domains
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99672dSp-VP64-p65(100-261)-RTA(75-190)Expresses dSp Cas9 fused to combination of truncated activation domains
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99673dSp-VP64-p65(100-261)-RTA(125-190)Expresses dSp Cas9 fused to combination of truncated activation domains
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99674dSp-VP64-p65(150-261)-RTA(125-190)Expresses dSp Cas9 fused to combination of truncated activation domains
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99675dSp-VP64-RTAExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domains
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99679pAAV-dSa-VP64-p65(100-261)-RTA(125-190)Expresses dSa Cas9 fused to optimized combination of truncated activation domains
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99680pAAV-dSa-VP64Expresses dSa Cas9 fused to gold standard VP64 activator
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99685pAAV-SCP1-dSa-VPR mini.Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter
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99686pAAV-EFS-dSa-VPR mini.Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
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99687pAAV-CMV-dSa-VPR mini.-1X snRP1Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal
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99688pAAV-CMV-dSa-VPR mini.-2X snRP1Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal
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99689pAAV-CMV-dSa-VPR mini.-syn pAExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal
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99690pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
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99691pAAV-CMV-dSa VP64 Actc1Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
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99692pAAV-SCP1-dSa VPR mini.-2X snRP-1 HbbExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
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99693pAAV-CMV-dSa VP64 HbbExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
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99694pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
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99695pAAV-CMV-dSa VP64 Neurog2Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
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99696pAAV-SCP1-dSa VPR mini.-2X snRP-1 AfpExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
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99697pAAV-CMV-dSa VP64 AfpExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV
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99698pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNAExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
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