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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp
(Plasmid #99696)

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  • Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
  • Depositing Lab
    George Church
  • Depositing Organization
    Harvard Medical School
    Located in United States of America
  • Publication
    Church Lab CRISPR plasmids 2017 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99696 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV (pUC f1)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9 and gRNA targeting Afp
  • Species
    Synthetic
  • Mutation
    dead Cas9
  • Entrez Gene
    Afp
  • Tag / Fusion Protein
    • VPR mini (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp was a gift from George Church (Addgene plasmid # 99696 ; http://n2t.net/addgene:99696 ; RRID:Addgene_99696)
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