Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM.
Demeautis C, Sipieter F, Roul J, Chapuis C, Padilla-Parra S, Riquet FB, Tramier M
Sci Rep. 2017 Jan 20;7:41026. doi: 10.1038/srep41026. PubMed Article
Demeautis C, Sipieter F, Roul J, Chapuis C, Padilla-Parra S, Riquet FB, Tramier M
Sci Rep. 2017 Jan 20;7:41026. doi: 10.1038/srep41026. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99867 | pmTFP1-ShadowG | Measure FRET between mTFP1 and ShadowG |
| 99868 | pLSSmOrange-mKate2 | Measure FRET between LSSmOrange and mKate2 |
| 99869 | AKARdual | Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate |
| 99870 | EKARdual | Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate |