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AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
Chow RD, Guzman CD, Wang G, Schmidt F, Youngblood MW, Ye L, Errami Y, Dong MB, Martinez MA, Zhang S, Renauer P, Bilguvar K, Gunel M, Sharp PA, Zhang F, Platt RJ, Chen S
Nat Neurosci. 2017 Aug 14. doi: 10.1038/nn.4620.

Plasmids from Article

ID Plasmid Purpose  
100275pAAV-U6sgp53-U6sgbbSapI-GFAPCreAAV-CRISPR mediated genome editing in glia / astrocytes
100276pAAV-U6sgp53-mTSG-GFAPCreAAV-CRISPR library for pool mutagenesis of top tumor suppressor genes
100277pLenti-U6sgbbBsmbI-puro-2A-FlucLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging