Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells.
Ishida K, Xu H, Sasakawa N, Lung MSY, Kudryashev JA, Gee P, Hotta A
Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1038/s41598-017-18568-4. PubMed Article
Ishida K, Xu H, Sasakawa N, Lung MSY, Kudryashev JA, Gee P, Hotta A
Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1038/s41598-017-18568-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100596 | pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro) | piggyBac vector expressing Dual-regulated Cas9 (Puro resistance) |
| 100597 | pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro) | piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance) |
| 100598 | pPV-H1-ccdB-mEF1α-RiH | piggyBac vector for sgRNA cloning |