pPV-H1-ccdB-mEF1α-RiH
(Plasmid
#100598)
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Purpose(Empty Backbone) piggyBac vector for sgRNA cloning
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPV piggyBac vector
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Backbone manufacturerHotta Lab at CiRA, Kyoto University
- Backbone size (bp) 7779
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Modifications to backbonepUC19 based piggyBac vector backbone with a cloning site (BamHI-EcoRI) for sgRNA or shRNA.
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Vector typeMammalian Expression ; piggyBac vector
- Promoter Human H1 PolIII promoter, human FTH1 promoter, mouse EEF1A1 intronic enhancer
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Selectable markersHygromycin ; mRFP1 fluorescent protein
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The piggyBac transposase can be obtained directly from Transposagen
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPV-H1-ccdB-mEF1α-RiH was a gift from Akitsu Hotta (Addgene plasmid # 100598 ; http://n2t.net/addgene:100598 ; RRID:Addgene_100598) -
For your References section:
Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells. Ishida K, Xu H, Sasakawa N, Lung MSY, Kudryashev JA, Gee P, Hotta A. Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1038/s41598-017-18568-4. 10.1038/s41598-017-18568-4 PubMed 29321585
Map uploaded by the depositor.
