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pPV-H1-ccdB-mEF1α-RiH
(Plasmid #100598)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100598 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPV piggyBac vector
  • Backbone manufacturer
    Hotta Lab at CiRA, Kyoto University
  • Backbone size (bp) 7779
  • Modifications to backbone
    pUC19 based piggyBac vector backbone with a cloning site (BamHI-EcoRI) for sgRNA or shRNA.
  • Vector type
    Mammalian Expression ; piggyBac vector
  • Promoter Human H1 PolIII promoter, human FTH1 promoter, mouse EEF1A1 intronic enhancer
  • Selectable markers
    Hygromycin ; mRFP1 fluorescent protein

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

The piggyBac transposase can be obtained directly from Transposagen

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPV-H1-ccdB-mEF1α-RiH was a gift from Akitsu Hotta (Addgene plasmid # 100598 ; http://n2t.net/addgene:100598 ; RRID:Addgene_100598)
  • For your References section:

    Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells. Ishida K, Xu H, Sasakawa N, Lung MSY, Kudryashev JA, Gee P, Hotta A. Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1038/s41598-017-18568-4. 10.1038/s41598-017-18568-4 PubMed 29321585