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RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a.
Tambe A, East-Seletsky A, Knott GJ, Doudna JA, O'Connell MR
Cell Rep. 2018 Jul 24;24(4):1025-1036. doi: 10.1016/j.celrep.2018.06.105.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
100817pDuBir-Lbu-dCas13a-avitagDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a
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