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pDuBir-Lbu-dCas13a-avitag
(Plasmid #100817)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100817 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDuBir
  • Backbone size w/o insert (bp) 7484
  • Total vector size (bp) 10961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
  • Alt name
    C2c2
  • Alt name
    WP_015770004 hypothetical protein [Leptotrichia buccalis]
  • Species
    Leptotrichia buccalis
  • Insert Size (bp)
    3477
  • Mutation
    R472A, H477A, R1048A, H1053A
  • GenBank ID
    WP_015770004.1 ACV39665.1
  • Promoter T7
  • Tags / Fusion Proteins
    • His6-MBP (N terminal on insert)
    • AviTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AarI (destroyed during cloning)
  • 3′ cloning site AarI (destroyed during cloning)
  • 5′ sequencing primer MBP_forward
  • 3′ sequencing primer T7_terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDuBir-Lbu-dCas13a-avitag was a gift from Mitchell O'Connell (Addgene plasmid # 100817 ; http://n2t.net/addgene:100817 ; RRID:Addgene_100817)
  • For your References section:

    RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a. Tambe A, East-Seletsky A, Knott GJ, Doudna JA, O'Connell MR. Cell Rep. 2018 Jul 24;24(4):1025-1036. doi: 10.1016/j.celrep.2018.06.105. 10.1016/j.celrep.2018.06.105 PubMed 30044970