pDuBir-Lbu-dCas13a-avitag
(Plasmid #100817)

• Purpose: Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a
• Depositing Lab: Mitchell O'Connell
• Publication: Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. doi: 10.1016/j.celrep.2018.06.105.

Backbone

• Vector backbone: pDuBir
• Backbone size w/o insert (bp): 7484
• Total vector size (bp): 10961
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lbu_Cas13a_R472A_H477A_R1048A_H1053A
• Alt name: C2c2
• Alt name: HEPN-nuclease deactivated LbuCas13a
• Alt name: WP_015770004 hypothetical protein [Leptotrichia buccalis]
• Species: Leptotrichia buccalis
• Insert Size (bp): 3477
• Mutation: R472A, H477A, R1048A, H1053A
• GenBank ID: WP_015770004.1 ACV39665.1
• Promoter: T7
• Tags / Fusion Proteins:
  • His6-MBP (N terminal on insert)
  • AviTag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AarI (destroyed during cloning)
• 3′ cloning site: AarI (destroyed during cloning)
• 5′ sequencing primer: MBP_forward
• 3′ sequencing primer: T7_terminator

Resource Information

• Supplemental Documents:
  • AviTag_pDuBir_AarI_ins_Lbu_ddC2c2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry