Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.
Le LTM, Nyengaard JR, Golas MM, Sander B
Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. PubMed
Le LTM, Nyengaard JR, Golas MM, Sander B
Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101651 | pGS_N_Strep | Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells |
| 101652 | pGS_C_Strep | Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells |
| 101653 | pGS_C_3xFLAG | Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells |
| 101654 | pGS_N_3xFLAG | Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells |
| 109191 | pGS-CD64-2 | Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system |
| 109192 | pGS-FCER1G-1 | Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system |