pGS-FCER1G-1
(Plasmid
#109192)
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PurposeEncodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIDS
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Backbone manufacturerGeneva Biotech
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman full-length FCER1G, codon-optimized
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Alt namehuman Fc receptor γ-chain FCER1G; high-affnity immunoglobulin epsilon receptor subunit gamma chain
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SpeciesH. sapiens (human)
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Insert Size (bp)261
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Entrez GeneFCER1G (a.k.a. FCRG)
- Promoter p10
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer p10
- 3′ sequencing primer HSV_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGS-FCER1G-1 was a gift from Monika Golas (Addgene plasmid # 109192 ; http://n2t.net/addgene:109192 ; RRID:Addgene_109192) -
For your References section:
Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain. Le LTM, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. 10.1007/s12033-017-0041-8 [pii] PubMed 29143175
