Escherichia coli amino acid auxotrophic expression host strains for investigating protein structure-function relationships.
Iwasaki T, Miyajima-Nakano Y, Fukazawa R, Lin MT, Matsushita SI, Hagiuda E, Taguchi AT, Dikanov SA, Oishi Y, Gennis RB
J Biochem. 2021 Apr 29;169(4):387-394. doi: 10.1093/jb/mvaa140. PubMed Article
Iwasaki T, Miyajima-Nakano Y, Fukazawa R, Lin MT, Matsushita SI, Hagiuda E, Taguchi AT, Dikanov SA, Oishi Y, Gennis RB
J Biochem. 2021 Apr 29;169(4):387-394. doi: 10.1093/jb/mvaa140. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 102799 | RF15 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted. |
| 102800 | RF16 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted. |
| 102801 | RF17 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted. |
| 102802 | RF18 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted. |
| 102803 | RF21 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC) |
| 102804 | EH1 | BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted. |