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Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ, Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y, Tuveson DA, Vakoc CR
Cell. 2017 Aug 24;170(5):875-888.e20. doi: 10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105505MSCV-Neo-GFP/emptyEmpty backbone of MSCV-driven retroviral cDNA expression
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105506MSCV-Neo-GFP/Foxa1MSCV-driven retroviral Foxa1 expression
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105507MSCV-Neo-GFP/V5-Foxa1MSCV-driven retroviral Foxa1 expression (V5-tagged)
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105508MSCV-Neo-GFP/Flag-Gata5MSCV-driven retroviral Gata5 expression (Flag-tagged)
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105509MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)
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105510LRG/Rosa26Lentiviral expression of Rosa26 targeting sgRNA
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105511LRG/Foxa1 e2.4Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA
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Antibodies from Article