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Ryan Mehl Lab: Bacterial expression plasmids for genetic code expansion laboratory course
Unpublished

Plasmids from Article

ID Plasmid Purpose  
105665pBad-CAexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag
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105666pBad-CA TAG20expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag
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105667pBad-CA TAG93expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tag
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105668pBad-CAts TAG97expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
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105836pBad-CA TAG126Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag
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105837pBad-CA TAG186Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
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105838pBad-CA TAG233Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag
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105839pBad-HPIIexpresses E. coli HPII catalase with N-terminal 6x his tag
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105843pBad-HPII TAG283expresses E. coli HPII catalase with TAG stop codon at site 283 and N-terminal 6x his tag
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105844pBad-HPII TAG348expresses E. coli HPII catalase with TAG stop codon at site 348 and N-terminal 6x his tag
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105845pBad-HPII TAG568expresses E. coli HPII catalase with TAG stop codon at site 568 and N-terminal 6x his tag
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105846pBad-HPII TAG206expresses E. coli HPII catalase with TAG stop codon at site 206 and N-terminal 6x his tag
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105847pBad-HPII TAG415expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag
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105848pBad-HPII TAG392expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag
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