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Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer.
Iwata F, Hirakawa H, Nagamune T
Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
107063pET15T-MsePCNA1Expresses M. sedula PCNA1 in E. coli
107064pET15T-MsePCNA2Expresses M. sedula PCNA2 in E. coli
107065pET15T-MsePCNA3Expresses M. sedula PCNA3 in E. coli
107069pMAL-SsoPCNA3Expresses S. solfataricus PCNA3 fused to MBP in E. coli
107071pET28b-MseLig1Expresses M. sedula DNA Ligase 1 in E. coli
107072pET15b-YCHost vector to construct a YPer or CyPet fusion protein
107073pMD-MsePCNA1M. sedula PCNA1
107074pMD-MsePCNA2M. sedula PCNA2
107075pMD-MsePCNA3M. sedula PCNA3
107076pMD-SsoPCNA1S. solfataricus PCNA1
107077pMD-SsoPCNA2S. solfataricus PCNA2
107078pMD-SsoPCNA3S. solfataricus PCNA3
107079pET15b-YM1M. sedula PCNA1 fused at the C-terminus of YPet
107080pET15b-YM2M. sedula PCNA2 fused at the C-terminus of YPet
107081pET15b-YM3M. sedula PCNA3 fused at the C-terminus of YPet
107082pET15b-YS1S. solfataricus PCNA1 fused at the C-terminus of YPet
107084pET15b-YS3S. solfataricus PCNA3 fused at the C-terminus of YPet
107085pET15b-M1CM. sedula PCNA1 fused at the N-terminus of CyPet
107086pET15b-M2CM. sedula PCNA2 fused at the N-terminus of CyPet
107087pET15b-M3CM. sedula PCNA3 fused at the N-terminus of CyPet
107088pET15b-S1CS. solfataricus PCNA1 fused at the N-terminus of CyPet
107090pET15b-S3CS. solfataricus PCNA3 fused at the N-terminus of CyPet

Antibodies from Article