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Erik Procko Lab: Procko Lab SERT constructs
Unpublished

Plasmids from Article

ID Plasmid Purpose  
107456pCEP4-SERT-mycMammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2
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107457pCEP4-DAT-mycMammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2
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107458pCEP4-NET-mycMammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2
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107459pCEP4-SERTG56A -mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype
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107460pCEP4-SERTA169D-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A169D conferring gain of function phenotype, decreased surface expression
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107461pCEP4-SERTA331T-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A331T conferring gain of function phenotype
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107462pCEP4-SERTS375L-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtype
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107463pCEP4-SERTG384S-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtype
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107464pCEP4-SERTI425V-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype
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107465pCEP4-SERTI425L-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425L conferring approximately wildtype transport phenotype, decreased surface expression
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107466pCEP4-SERTT439M-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtype
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107467pCEP4-SERTT448K-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T448K conferring gain of function phenotype, decreased surface expression
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107468pCEP4-SERTR464Q-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R464Q approximately wildtype transport ability
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107469pCEP4-SERTF465L-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotype
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107470pCEP4-SERTS477F-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expression
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107471pCEP4-SERTG484E-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtype
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107472pCEP4-SERTL550V-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L550V approximately wildtype transport
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107473pCEP4-SERTI576V-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576V approximately wildtype transport
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107474pCEP4-SERTN177E-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activity
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107475pCEP4-SERTK605N-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expression
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107476pCEP4-SERTK37Q-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K37Q approximately wildtype activity
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107477pCEP4-SERTR79V-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R79V conferring gain of function phenotype, decreased surface expression
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107478pCEP4-SERTF88H-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F88H conferring gain of function phenotype
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107479pCEP4-SERTA173G-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A173G with approximately wildtype activity, decreased surface expression
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107480pCEP4-SERTY289I-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activity
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107481pCEP4-SERTT233F-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T233F with approximately wildtype activity
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107482pCEP4-SERTF263C-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F263C with approximately wildtype activity, decreased surface expression
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107483pCEP4-SERTR596Q-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotype
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107484pCEP4-SERTV343T-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation V343T conffering gain of function phenotype, decreased surface expression
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107485pCEP4-SERTI379Q-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I379Q conferring gain of function phenotype, decreased surface expression
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107486pCEP4-SERTE392M-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E392M with approximately wildtype activity
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107487pCEP4-SERTL406I-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L406I with approximately wildtype activity
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107488pCEP4-SERTY410G-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y410G with approximately wildtype activity, decreased surface expression
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107489pCEP4-SERTR462E-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expression
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107490pCEP4-SERTL467D-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L467D with approximately wildtype activity, decreased surface expression
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107491pCEP4-SERTC473H-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation C473H conferring gain of function phenotype
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107492pCEP4-SERTK490L-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K490L conferring gain of function phenotype, decreased surface expression
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107493pCEP4-SERTE494Y-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E494Y conferring gain of function phenotype
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107494pCEP4-SERTP499K-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation P499K conferring gain of function phenotype
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107495pCEP4-SERTF556H-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F556H conferring gain of function phenotype
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107496pCEP4-SERTI576K-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expression
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107497pCEP4-SERTG582K-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expression
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107498pCEP4-SERTS585P-mycMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype
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Recombinant Antibodies from Article