Local control of intracellular microtubule dynamics by EB1 photodissociation.
van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann T
Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 10.1038/s41556-017-0028-5.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107614 | pEB1N-LZ-LOV2(wt) | N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag |
| 107690 | pEB1N-LZ-LOV2(fast) | N-terminal half of π-EB1 with fast LOV2, no fluorescent tag |
| 107693 | pEB1N-LZ-mCherry-LOV2(wt) | N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends |
| 107695 | pmCherry-Zdk1-EB1C | C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light |
| 107696 | pEGFP-Zdk1-EB1C | C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light |
| 107726 | pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1 | Knock out of EB1 in human cells by CRISPR/Cas9 |
| 107727 | pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2 | Knock out of EB1 in human cells by CRISPR/Cas9 |
| 107728 | pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3 | Knock out of EB1 in human cells by CRISPR/Cas9 |
| 107729 | pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1 | Knock out of EB3 in human cells by CRISPR/Cas9 |
| 107730 | pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2 | Knock out of EB3 in human cells by CRISPR/Cas9 |
| 107731 | pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3 | Knock out of EB3 in human cells by CRISPR/Cas9 |