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The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes.
Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC
Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840. Epub 2018 Feb 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
107938pCFJ150-GFP(dpiRNA)::CDK-1cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.
107939pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
107940pCFJ150-Cas9(dpiRNA)eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Antibodies from Article