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pCFJ150-Cas9(dpiRNA)
(Plasmid #107940)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107940 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9(dpiRNA)
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    4277
  • Promoter eft-3

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGTTTTTTTTTCAGTTGGGAAACACCC
  • 3′ sequencing primer gagaagaagggaatgcttgaaagg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ150-Cas9(dpiRNA) was a gift from Heng-Chi Lee (Addgene plasmid # 107940)
  • For your References section:

    The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes. Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC. Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840. Epub 2018 Feb 1. 10.1126/science.aao2840 PubMed 29420292