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Addgene
Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences.
Boehm V, Gerbracht JV, Marx MC, Gehring NH
Nat Commun. 2016 Dec 5;7:13691. doi: 10.1038/ncomms13691. PubMed Article

Plasmids from Article

ID Plasmid Purpose
108366pCI-globin_del5UTR_WT-xrRNA-4HExpresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108367pCI-globin_del5UTR_PTC39-xrRNA-4HExpresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108368pCI-TPI_WT-xrRNA-4HExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108369pCI-TPI_PTC160-xrRNA-4HExpresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108370pCI-TPI_WT-xrRNA-4MS2-4HExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)
108371pCI-TPI_WT-4MS2-xrRNA-4HExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)
108372pCI-TPI_WT-RAB7A_3UTR-xrRNA-4HExpresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108373pCI-TPI_WT-IL6_3UTR-xrRNA-4HExpresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108374pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4HExpresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108375pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4HFRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108376pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4HFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108377pcDNA5/FRT/TO-TPI_WT-xrRNA-4HFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108378pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4HFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

Antibodies from Article