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A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed.
Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6.
PubMed

Plasmids from Article

ID Plasmid Purpose  
108389AF20-double-tagExpress human PC1
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108390pcDNA3-mPC1_4077-eGFP-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293
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108391pcDNA3-mPC1_4077-eGFPExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293
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108392pcDNA3-mPC1_4077-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4077 to 4293
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108393pcDNA3-mPC1_4119-eGFP-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4119 to 4293
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108394pcDNA3-mPC1_4119-eGFPGenomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293
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108395pcDNA3-mPC1_4119-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4119 to 4293
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108396pcDNA3-mPC1_4148-eGFP-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4148 to 4293
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108397pcDNA3-mPC1_4148-eGFPExpress cytoplasmic tail mouse PC1 from amino acid 4148 to 4293
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108398pcDNA3-mPC1_4148-3HAExpress cytoplasmic tail mouse PC1 from amino acid 4148 to 4293
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108399pcDNA3-hPC1_4106-eGFP-3HAExpress cytoplasmic tail human PC1 from amino acid 4106 to 4302
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108401pcDNA3-CD16.7-hPC1-eGFP-3HAExpress cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor
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108402pcDNA3-hPC1_MTS-eGFPExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157
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108403pcDNA3-hPC1_MTS_L4137P-eGFPExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.
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108404pcDNA3-hPC1_MTS_L4139P-eGFP-3HAExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.
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108405pcDNA3-hPC1_MTS_V4146I-eGFPExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.
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108406pcDNA5-eGFP-PC1-3HACan be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.
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Antibodies from Article