Addgene: protaTETHER - a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc-GFP fusion protein.

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Robert Hughes
Norris et al

protaTETHER - a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc-GFP fusion protein.
Norris JL, Hughes RM
FEBS Open Bio. 2018 Apr 25;8(6):1029-1042. doi: 10.1002/2211-5463.12414. eCollection 2018 Jun. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
108579	PKAcs-nolinker-GFP	Expresses PKAcs-GFP fusion in mammalian cells
108583	PKAcs-GGS4X-GFP	Expresses PKAcs-GFP fusion in mammalian cells with flexible linker
108584	PKAcs-GGSD5X-GFP	Expresses PKAcs-GFP fusion in mammalian cells with flexible linker
108585	PKAcs-KR6X-GFP	Expresses PKAcs-GFP fusion in mammalian cells with structured linker
108587	PKAcs-L10X-GFP	Expresses PKAcs-GFP fusion in mammalian cells with structured linker
108588	PKAcs-G12X-GFP	Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

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