PKAcs-GGS4X-GFP
(Plasmid
#108583)
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PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linker
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Depositing Lab
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Depositing OrganizationEast Carolina University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 108583-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 108583-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonephCMV-GFP
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Backbone manufacturerGenlantis
- Backbone size w/o insert (bp) 3909
- Total vector size (bp) 4962
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameprotein kinase A, catalytic subunit
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SpeciesH. sapiens (human)
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Insert Size (bp)1053
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MutationH87Q and W196R
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPKA catalytic subunit was a gift from Susan Taylor.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 108583-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 108583-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKAcs-GGS4X-GFP was a gift from Robert Hughes (Addgene plasmid # 108583 ; http://n2t.net/addgene:108583 ; RRID:Addgene_108583) -
For your References section:
protaTETHER - a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc-GFP fusion protein. Norris JL, Hughes RM. FEBS Open Bio. 2018 Apr 25;8(6):1029-1042. doi: 10.1002/2211-5463.12414. eCollection 2018 Jun. 10.1002/2211-5463.12414 PubMed 29928581
