Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies.
Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander D
Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 10.1073/pnas.1710617115. Epub 2018 Feb 20. PubMed Article
Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander D
Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 10.1073/pnas.1710617115. Epub 2018 Feb 20. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110760 | ISG15 C-term. Domain intein/chitin binding domain (79-156) | Bacterial expression of ISG15 C-term. |
| 110761 | ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154) | Bacterial expression of proISG15 |
| 110762 | proISG15 (2-165) | Bacterial expression of proISG15 |
| 110763 | proISG15 C-term. domain (79-165) | Bacterial expression of proISG15 C-term. domain |
| 110764 | mature ISG15 C-term. domain (79-157) | Bacterial expression of proISG15 C-term. domain |
| 110765 | mature ISG15 C-term. Domain (79-165, W123A) | Bacterial expression of mutant proISG15 C-term. domain |
| 110766 | Met1 diUb (1-152) | Bacterial expression of Met1 diUb |
| 110767 | Met1 diUb (1-152, R42W) | Bacterial expression of Met1 diUb |