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Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies.
Swatek KN, Aumayr M, Pruneda JN, Visser LJ, Berryman S, Kueck AF, Geurink PP, Ovaa H, van Kuppeveld FJM, Tuthill TJ, Skern T, Komander D
Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2371-2376. doi: 10.1073/pnas.1710617115. Epub 2018 Feb 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110760ISG15 C-term. Domain intein/chitin binding domain (79-156)Bacterial expression of ISG15 C-term.
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110761ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)Bacterial expression of proISG15
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110762proISG15 (2-165)Bacterial expression of proISG15
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110763proISG15 C-term. domain (79-165)Bacterial expression of proISG15 C-term. domain
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110764mature ISG15 C-term. domain (79-157)Bacterial expression of proISG15 C-term. domain
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110765mature ISG15 C-term. Domain (79-165, W123A)Bacterial expression of mutant proISG15 C-term. domain
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110766Met1 diUb (1-152)Bacterial expression of Met1 diUb
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110767Met1 diUb (1-152, R42W)Bacterial expression of Met1 diUb
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Recombinant Antibodies from Article