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Rapid production of pure recombinant actin isoforms in Pichia pastoris.
Hatano T, Alioto S, Roscioli E, Palani S, Clarke ST, Kamnev A, Hernandez-Fernaud JR, Sivashanmugam L, Chapa-Y-Lazo B, Jones AME, Robinson RC, Sampath K, Mishima M, McAinsh AD, Goode BL, Balasubramanian MK
J Cell Sci. 2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111146pPICZc-HsACTB*-thymosinB-8HisExpresses human beta-actin fused with thymosin beta and a His tag.
111147pPICZc-HsACTG1*-thymosinB-8HisExpresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
111148pPICZc-ScAct1*-thymosinB-8HisExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37
111149pPICZc-Ub-R-HsACTB*-thymosinB-8HisExpresses arginylated human beta-actin fused with thymosin beta and a His tag.

Antibodies from Article