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In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology.
Fazli M, Harrison JJ, Gambino M, Givskov M, Tolker-Nielsen T
Appl Environ Microbiol. 2015 Jun;81(11):3623-30. doi: 10.1128/AEM.03909-14. Epub 2015 Mar 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111608pDONRPEX18Tp-SceI-pheSGateway compatible allelic exchange vector for Burkholderia sp.
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111609pDONRPEX18Gm-SceI-pheSGateway compatible allelic exchange vector for Burkholderia sp.
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111610pDONRPEX18Km-SceI-pheSGateway compatible allelic exchange vector for Burkholderia sp.
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111611pDAI-SceI-pheSYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.
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Antibodies from Article