Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation.
Tang W, Hu JH, Liu DR
Nat Commun. 2017 Jun 28;8:15939. doi: 10.1038/ncomms15939. PubMed Article
Tang W, Hu JH, Liu DR
Nat Commun. 2017 Jun 28;8:15939. doi: 10.1038/ncomms15939. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 96849 | pWT016g | processed HHR-sgRNA-GFP |
| 96850 | pWT016f | HHR-bsgRNA-GFP (10 nt blocker) |
| 96851 | pWT016e | dHHR-bsgRNA-GFP (10 nt blocker) |
| 96852 | pWT016a | HHR-bsgRNA-GFP (13 nt blocker) |
| 96853 | pWT016b | dHHR-bsgRNA-GFP (13 nt blocker) |
| 96854 | pWT016h | HHR-bsgRNA-GFP (17 nt blocker) |
| 96855 | pWT016d | dHHR-bsgRNA-GFP (17 nt blocker) |
| 96856 | pWT016i | theophylline-agRNA-GFP |
| 96857 | pWT016l | (d)theophylline-agRNA-GFP |
| 96858 | pWT029c | guanine-agRNA (GFP activation) |
| 96859 | pWT029d | (d)guanine _agRNA (GFP activation) |
| 96860 | pWT029g | guanine-agRNA (GFP activation, 18 nt blocker with bulges) |
| 96861 | pWT029h | (d)guanine-agRNA (GFP activation,18 nt blocker with bulges) |
| 96862 | pWT049d | guanine-agRNA (19 nt blocker with bulges, RFP activation) |
| 96863 | pWT049e | (d)guanine-agRNA (19 nt blocker with bulges, RFP activation) |
| 96864 | pWT055g | sgRNA-HEK3 |
| 96865 | pWT055f | sgRNA-FANCF |
| 96866 | pWT055e | sgRNA-EMX1 |
| 96867 | pWT055h | sgRNA-HEK4 |
| 96868 | pWT057e | HHR-bsgRNA-HEK3 |
| 96869 | pWT057f | dHHR-bsgRNA-HEK3 |
| 96870 | pWT057c | HHR-bsgRNA-FANCF |
| 96871 | pWT057d | dHHR-bsgRNA-FANCF |
| 96872 | pWT057a | HHR-bsgRNA-EMX1 |
| 96873 | pWT057b | dHHR-bsgRNA-EMX1 |
| 96874 | pWT057g | HHR-bsgRNA-HEK4 |
| 96875 | pWT057h | dHHR-bsgRNA- HEK4 |
| 96876 | pWT055c | theophylline-agRNA-HEK3 |
| 96877 | pWT055b | theophylline-agRNA-FANCF |
| 96878 | pWT015a | GFP reporter |
| 96879 | pWT032a | RFP reporter |