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Modular fluorescence complementation sensors for live cell detection of epigenetic signals at endogenous genomic sites.
Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A
Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
191392Flag-NLS-MaSat ZF-18-mVenusNZinc finger fused to N-terminal part of split mVenus
191393pcDNA3 Flag-MBD1-14-mVenusCMBD domain of MBD1 fused to C-terminal part of split mVenus
191394pcDNA3 Flag-MBD1 R44Q-14-mVenusCR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus
191395pTH-PanCen TALE-mVenusNTALE fused to N-terminal part of split mVenus
191396pHAGE-TO-Sp dCas9-mVenusNSpdCas9 fused to N-terminal part of mVenus
191397pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-NeosgRNA targeting alpha-satellites on human chromosome 9
191398FLAG-mVenusC-14-HP1b ChromoHP1b chromo domain fused to C-terminal part of split mVenus
191399FLAG-mVenusC-14-HP1b Chromo W42AW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Antibodies from Article