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Wide-area all-optical neurophysiology in acute brain slices.
Farhi SL, Parot VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou S, Jun Kim J, Campbell RE, Cox DD, Cohen AE
J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0168-19.2019. doi: 10.1523/JNEUROSCI.0168-19.2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
102995pAAV-synp-F-H2B-jRGECO1aExpresses histone fused jRGECO1a under the synapsin promoter
112278SLF155: AAV-syn-DO-H2B-jRGECO1aFor AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch
112280SLF045: AAV-hSyn-eTsChR-GFPAAV based broad expression of eTsChR in neurons
112281SLF091: Lenti-CaMKIIa-eTsChR-eGFPFor lentiviral or direct expression of eTsChR in excitatory neurons
112282SLF060: Lenti-CaMKIIa-jRGECO1aFor lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons
124775SLF063: Lenti-fCAG-jRGECO1aFor general lentiviral or direct expression of cytosolic jRGECO1a
124776SL016: AAV-CAG-FLEX-CheRiff-GFPFor AAV based expression of CheRiff in Cre-Positive cells

Antibodies from Article