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RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs.
Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 10.1093/nar/gkx801. PubMed Article

Plasmids from Article

ID Plasmid Purpose
112705pcDNA3-FLAG-RNMTExpresses N-terminally FLAG-tagged human RNMT in mammalian cells
112706pcDNA3-FLAG-RNMT 1-120Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells
112707pcDNA3-FLAG-RNMT 121-476Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells
112708pcDNA3-ΔN-RNMTExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
112709pGEX-2T-GST-RNMTExpresses N-terminally GST-tagged human RNMT in bacterial cells
112710pET28b-His-hCEExpresses N-terminally His-tagged human CE/RNGTT in bacterial cells
112818pGEX-2T-GST-eIF4E K119AExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Antibodies from Article