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A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold.
Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, Moldavski O, Hurley JH, Zoncu R
Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 10.1038/s41556-018-0148-6. Epub 2018 Jul 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112740pLJM1 FRB-myc-RagA express FRB-myc-RagA
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112741pLJM1 FRB-myc-RagC express FRB-myc-RagC
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112742pLJM1 TMEM192-Flag express pLJM1 TMEM192-Flag
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112743pLJM1 TMEM192-Flag-FKBP express TMEM192-Flag-FKBP
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112744pLJM1 Raptor GFP express Raptor-GFP
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112749pRK5 GFP RagB Q99L express GFP-RagB (Q99L mutant)
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112750pRK5 GST MetaP2express HA-GST-FRB-MetaP2
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112752pRK5 Flag RagC (D181N) express Flag-RagC (D181N mutant)
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112753pRK5 Halo RagB express Halo-RagB
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112754pEGFP-N1 Raptor Haloexpress Raptor-Halo
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112755pRK5 Flag RagBexpress Flag-RagB
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112756pLVX Flag RagC WTexpress Flag-RagC
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112757pLVX Flag RagC S75Nexpress Flag-RagC (S75N mutant)
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112758pLVX Flag RagC T90Nexpress Flag-RagC (T90N mutant)
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112759pLVX Flag RagC W115Rexpress Flag-RagC (W115R mutant)
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112760pLJM1 Flag GFP 4E-BP1 Omp25express GFP-4EBP1-Omp25
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112761pLJM1 Flag GFP 4E-BP1 Rheb15express GFP-4EBP1-Rheb15
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112762pLJM1 Flag GFP 4E-BP1 Rheb15 CAAXexpress GFP-4EBP1-Rheb15(CAAX mutation)
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112764pRK5 Myc Rheb Omp25express myc-Rheb-Omp25
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112765pRK5 GFP RagB (GD)express GFP-RagB (G domain only)
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112766pRK5 GFP RagB (CTD)express GFP-RagB (C terminal domain only)
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112767pRK5 GFP RagC (GD)express GFP-RagC (G domain only)
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112768pRK5 GFP RagC (CTD)express GFP-RagC (C terminal domain only)
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112769pRK5 Flag RagB (CTD)express Flag-RagB (C terminal domain only)
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112770pRK5 Flag RagB (GD)express Flag-RagB (G domain only)
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112771pRK5 Flag RagC (CTD)express Flag-RagC (C terminal domain only)
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112772pRK5 Flag RagC (GD)express Flag-RagC (G domain only)
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112773pRK5 Flag RagB(GD)/RagC(CTD) chimeraexpress Flag-RagB (Gdomain) fused with RagC (C terminal domain)
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112774pRK5 Flag RagC(GD)/RagB(CTD) chimeraexpress Flag-RagC (G domain) fused with with RagB (C terminal domain)
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